Journal: Translational oncology
Article Title: Connexin43 is associated with the progression of clear cell renal carcinoma and is regulated by tangeretin to sygergize with tyrosine kinase inhibitors.
doi: 10.1016/j.tranon.2023.101712
Figure Lengend Snippet: Fig. 8. (A) Tangeretin and sunitinib synergistically inhibited the colony formation of ACHN cells. (B) Knocking down Cx43 expression also reduced the EGFR phosphorylation and MMP-9 expression in ACHN cells. (C) Tangeretin synergistically inhibited ERK phosphorylation in OS-RC-2 cells treated with sunitinib. (D) Knocking down Cx43 expression reduced EGFR phosphorylation and survivin expression in RCC OS-RC-2 cells. (E) Tangeretin inhibited STAT3 phosphorylation, survivin and Bcl-2 expression in 786-O cells treated with or without sunitinib. (F) Tangeretin inhibited Cx43 in 786-O cells, which is different from sunitinib. (G) Little diference between in tangeretin + Cx43-siRNA group and tangeretin + negative control group in ACHN cells. (H) Protein-protein interaction network of Cx43 and tangeretin target genes following Search Tool for the Retrieval of Interacting Genes/Proteins analysis. Since the molecular mass for the phosphorylated epitopes and their total protein were too close to run on the same blot, part of the sample in the Figure was repeated by another blot with their corresponding loading control, whilst the level of the phosphorylated epitopes were normalized to that of their corresponding proteins EGFR, ERK and STAT3 along with their loading controls. All cell experiments with statistical diagrams were repeated ≥three times. Tan, tangeretin; Su, sunitinib; Cx43, connexin 43; p- phosphorylation; NC, negative control; S3, siCx43_3.
Article Snippet: Cx43 (cat. no. 3512), EGFR (cat. no. 4267), phosphorylated (p)-EGFR (cat. no. 3777), ERK (cat. no. 4695), p-ERK (cat. no. 4370), α/β-tubulin (cat. no. 2148), MMP-9 (cat. no. 13,667), STAT3 (cat. no. 9139), Bcl-2 (cat. no. 15,071), survivin (cat. no. 2808) and p-STAT3 (cat. no. 9145) primary antibodies were acquired from Cell Signaling Technology, Inc.
Techniques: Expressing, Phospho-proteomics, Negative Control, Control
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